Device

Part:BBa_K142076:Design

Designed by: Julius Rabl   Group: iGEM08_ETH_Zurich   (2008-10-29)


lac-controlled RFP generator with constitutive lacI expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1084
    Illegal NheI site found at 1107
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2285
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We put the LacI generator behind the lac-controlled expression cassette to minimze readthrough.



Source

Subcloned from BioBricks using standard molecular biology techniques.


References